P-431 Effect of biphasic CAPA-IVM on ovarian tissue oocytes of transgender men

Abstract Study question Can CAPA-IVM (Biphasic in-vitro maturation) with C-type natriuretic peptide (CNP), followed by in vitro maturation (IVM) improve ovarian tissue oocyte (OTO) transgender patients? Summary answer Biphasic did not show any significant difference competence and genetic variations when compared in-house IVM. What is known already OTO-IVM a method of fertility preservation patients where prior stimulation undesired. It has resulted live births cancer polycystic syndrome (PCOS) patients. further improved the OTO competency these Similarly, could be collected from men without during gender reassignment surgery. Even having adequate survival maturation, exhibited decreased fertilization rate severely compromised developmental probably due to long testosterone treatment before For this reason, we sought investigate whether potential can rescued through biphasic CAPA-IVM. design, size, duration Patients were recruited July 2022 December 2022. Ovaries 4 (age= 18-26 years, mean age=21 years) who underwent surgery after (mean length= 32 months). All ovaries cold medium (4oC) manipulation was performed within 30 minutes collection for retrieval cumulus complexes (COCs). Participants/materials, setting, methods COCs cultured either IVM 48 hours or 54 hours. Following oocytes analyzed Calcium (Ca2+)-releasing ICSI. In matured GV (germinal vesicle), MI (metaphase I) vivo clusters smooth endoplasmic reticulum (SERa) served as controls. Genetic analysis on subsequent embryos detect chromosomal abnormalities all groups. Main results role chance A Total 133 (44 53 IVM) both showed similar i.e. 55%. After culture period, tended higher (78% vs. 68%, p = 0.184). ICSI, 8/11 8/13 normally fertilized, comparable controls (5/5) (p 0.195 0.103 respectively). Blastocyst rates lower (1/8) house (2/8) (3/5), however 0.071 0.207 Ca2+-releasing determined product amplitude frequency, arbitrary units (AU). The average values (1.61) (1.58) 0.97). Shallow whole genome sequencing (Shallow WGS) developed that 3/5 PMC group, 3/7 group chromosomally abnormal. Limitations, reasons caution number included low, so obtained should interpreted caution. Wider implications findings To best our knowledge, first study use oocytes. Further investigation added value together effect length might help shape more accurate guidelines men. Trial registration applicable